Antibodies |
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Bacterial and virus strains |
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Biological samples |
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Chemicals, peptides, and recombinant proteins |
EnGen Spy Cas9 NLS |
New England Biolabs |
M0646M |
EnGen Spy dCas9 (SNAP-Tag) |
New England Biolabs |
M0652T |
TrueCut Cas9 Protein v2 |
ThermoFisher |
A36499 |
Terminal Transferase |
Millipore Sigma |
3333574001 |
[ɣ-32P]-ATP (6000 Ci/mmole) |
Perkin Elmer |
BLU502Z250UC |
SYTOX Red Dead Cell Stain |
ThermoFisher |
S34859 |
Critical commercial assays |
QIAQuick PCR Purification Kit |
Qiagen |
28104 |
Lipofectamine CRISPRMAX Cas9 Transfection Reagent |
ThermoFisher |
CMAX00003 |
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Deposited data |
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Experimental models: Cell lines |
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Experimental models: Organisms/strains |
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Oligonucleotides |
Pools, primers, and other sequences |
This paper |
See Data.S2
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Biotinylated Oligo(dT) probe |
Promega |
Z5261 |
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Recombinant DNA |
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Software and algorithms |
FastAptamer |
FASTAptamer: A Bioinformatic Toolkit for Combinatorial Selections | Burke Lab | University of Missouri (Alam et al., 2015) |
N/A |
Geneious |
BioMatters Ltd. |
N/A |
BioRender |
BioRender |
N/A |
RNAfold 2.5.1 |
Lorenz et al., 2011
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N/A |
MAFFT 7 |
Katoh et al., 2019
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SciPy 1.9.0 |
Virtanen et al., 2020
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N/A |
Python 3.10.6 |
Van Rossum and Drake, 2009
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Matplotlib 3.5.3 |
Hunter, 2007
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pm_multi |
Hofacker et al., 2004
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Structure logo |
Gorodkin et al., 1997
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Kneed 0.7.0 |
Satopaa et al., 2011
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Seaborn 0.11.0 |
Waskom, 2021
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Other |
Amicon UltraCel 10, 0.5 Centrifugal Filter Unit |
Millipore-Sigma |
UFC501096 |
Amicon UltraCel 30, 0.5 Centrifugal Filter Unit |
Millipore-Sigma |
UFC503096 |
Whatman Protran BA 25 mm 0.45 micron nitrocellulose |
GE Healthcare Life Sciences |
10402506 |
Dynabeads MyOne Streptavidin C1 beads |
ThermoFisher |
65002 |
Micro Bio-Spin P-30 columns |
BioRad |
7326251 |
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