KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-GluRIIA (8B4D2) (1:50) | Developmental Studies Hybridoma Bank | RRID: AB_528269 |
| Rabbit anti-GluRIIB (1:1000) | Reference # Perry et al.60 | N/A |
| Guinea pig anti-GluRIID (1:1000) | Reference # Perry et al.60 | N/A |
| Alexa Fluor 488 conjugated secondary antibodies (1:400) | Jackson ImmunoResearch Laboratories (Jackson) | Cat#715-545-150; RRID: AB_2340846 |
| Cy3-conjugated secondary antibodies (1:400) | Jackson | Cat#706-165-148; RRID: AB_2340460, Cat#711-165-152; RRID: AB_2307443 |
| DyLight 405-conjugated secondary antibodies (1:400) | Jackson | Cat#706-475-148; RRID: AB_2340470, Cat#711-475-152; RRID: AB_2340616 |
| Experimental models: Organisms/strains | ||
| w 1118 | Bloomington Drosophila Stock Center (BDSC) | Cat#5905 |
| nos.Cas9-DsRed | BDSC | Cat#79004 |
| P{TKO.GS00444} (gRNA targeting GluRIIA) | BDSC | Cat#68059 |
| dHb9-Gal4 (Ib-Gal4) | BDSC | Cat#83004 |
| GMR2 7E09-Gal4 (Is-Gal4) | BDSC | Cat#49227 |
| UAS-RedStinger | BDSC | Cat#8547 |
| GluRIIA PV3 | This study | N/A |
| GluRIIA PV7 | This study | N/A |
| GluRIIB SP5 | This study | N/A |
| GluRIIB SP14 | This study | N/A |
| pU6-BbsI-{GluRIIB.gRNA} | This study | N/A |
| GluRIIA Q615R , GluRIIB SP6 | This study | N/A |
| LexAOp-vGlut | This study | N/A |
| GluRIIA SP22 | Reference # Diantonio et al.16 | N/A |
| GluRIIA Q615R | Reference # Perry et al.34 | N/A |
| OK371-GAL4 | Reference # Mahr et al.61 | N/A |
| UAS-vGlut | Reference # Daniels et al.18 | N/A |
| OK319-GAL4 | Reference # Sweeney et al.62 | N/A |
| G14-Gal4 | Reference # Aberle et al.63 | N/A |
| UAS-BoNT-C | Reference # Han et al.11 | N/A |
| SynapGCaMP8f | Reference # Han et al.11 | N/A |
| mnb 1 | Reference # Chen et al.28 | N/A |
| vGlut-LexA | Reference # Sherer et al.64 | N/A |
| GluRIIA mRFP | Reference # Qin et al.15 | N/A |
| UAS-GluRIIB | Reference # Perry et al.34 | N/A |
| GluRIIA-T2A-Gal4 | Reference # Perry et al.65 | N/A |
| GluRIIB-T2A-Gal4 | Reference # Kondo et al.65 | N/A |
| Recombinant DNA | ||
| pU6-BbsI-chiRNA | Addgene | Cat#45946 |
| pDEST-APLO | Addgene | Cat#112805 |
| pACU2 | Addgene | Cat#31223 |
| Software and algorithms | ||
| NIS Elements software | Nikon | 4.51.01 |
| Huygens Object Analysis | Scientific Volume Imaging | 22.04 |
| Axon pCLAMP Clampfit | Molecular Devices | 10.7 |
| MiniAnalysis | Synaptosoft | 6.0.3 |
| GraphPad Prism | GraphPad | 8.0.1 |
| ImageJ (Fiji) | Reference # Rueden et al.66 | N/A |