Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-Cdc13 antibody | Novus Biologicals | NB200-576 |
| Chemicals, peptides, and recombinant proteins | ||
| 1-NM-PP1 (4-Amino-1-tert-butyl-3-(1’-naphthylmethyl)pyrazolo[3,4-d]pyrimidine) | Toronto Research Chemicals | A603003 |
| Latrunculin B | Abcam | ab144291 |
| Experimental models: Organisms/strains | ||
| S. pombe JM366: WT 972 h- (Alternate name: PN1) | Lab collection | N/A |
| S. pombe JM6972: cdc13-yomNeonGreen::hphR lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1:[pJK148-Pact-yomRuby3-term] h+ | This Study | N/A |
| S. pombe JM7003: rga2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc13-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h- | This Study | N/A |
| S. pombe JM7008: rga4Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc13-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] | This Study | N/A |
| S. pombe JM7088: cdr2Δ::NAT lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc13-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h+ | This Study | N/A |
| S. pombe JM7171: lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc25-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h- | This Study | N/A |
| S. pombe JM7172: rga4Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc25-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h- | This Study | N/A |
| S. pombe JM6752: rga2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc25-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h- | This Study | N/A |
| S. pombe JM6753: cdr2Δ::NAT lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc25-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h- | This Study | N/A |
| S. pombe JM7492: lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1Δ::Pcdc13::cdc13-L-cdc2as::cdc13 3'UTR::ura4+ cdc2Δ::kanMX6 cdc13Δ::natMX6 h+ | This Study | N/A |
| S. pombe JM7490: rga2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1Δ::Pcdc13::cdc13-L-cdc2as::cdc13 3'UTR::ura4+ cdc2Δ::kanMX6 cdc13Δ::natMX6 | This Study | N/A |
| S. pombe JM7502: rga4Δ::hphR lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1Δ::Pcdc13::cdc13-L-cdc2as::cdc13 3'UTR::ura4+ cdc2Δ::kanMX6 cdc13Δ::natMX6 | This Study | N/A |
| S. pombe JM7479: lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1-32:[pJK148-Pwee1-wee1-Twee1] h+ | This Study | N/A |
| S. pombe JM7558: lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1-32:[pJK148-Pwee1-wee1-Twee1] rga4Δ::NAT | This Study | N/A |
| S. pombe JM7532: rga2Δ::hphR lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX leu1-32:[pJK148-Pwee1-wee1-Twee1] h+ | This Study | N/A |
| S. pombe JM7503: ppa2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX h+ | This Study | N/A |
| S. pombe JM7512: rga4Δ::hphR ppa2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | This Study | N/A |
| S. pombe JM7533: rga2Δ::hphR ppa2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX h+ | This Study | N/A |
| S. pombe JM7690: Diploid lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX / pil1-mCherry::natR | This Study | N/A |
| S. pombe JM7674: Diploid rga2Δ::NAT lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX / rga2Δ::hphR lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | This Study | N/A |
| S. pombe JM7630: cdr2Δ::NAT zfs1Δ::kanMX6 ppa2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | This Study | N/A |
| S. pombe JM7645: cdr2Δ::NAT rga4Δ::hphR zfs1Δ::kanMX6 ppa2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | This Study | N/A |
| S. pombe JM7646: cdr2Δ::NAT rga2Δ::hphR zfs1Δ::kanMX6 ppa2Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | This Study | N/A |
| S. pombe JM7627: ppa2Δ::kanMX6 zfs1Δ::kanMX6 | This Study | N/A |
| S. pombe JM7381: cdr2Δ::NAT lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | Lab collection | Opalko et al.33 |
| S. pombe JM6434: lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | Martin Lab AV1469 | Vještica et al.67 |
| S. pombe JM6963: cdr2Δ::NAT rga4Δ::kanMX6 lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc25-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] | This Study | N/A |
| S. pombe JM6831: rga2Δ::kanMX6 cdr2Δ::NAT lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX cdc25-yomNeonGreen::hphR leu1:[pJK148-Pact-yomRuby3-term] h- | This Study | N/A |
| S. pombe JM6614: cdc13-mCherry-kanMX | This Study | N/A |
| S. pombe JM7701: cdc13+-internal-sfGFPcp | Hauf lab ST947 | Kamenz et al.46 |
| S. pombe JM6713: cdc13-mNeonGreen-hphR h- | This Study | N/A |
| S. pombe JM5290: cdc25-yomNeonGreen::hphR h+ | This Study | N/A |
| S. pombe JM656: sec8-1 ura4-D18 leu1-32 h+ | Lab collection | N/A |
| S. pombe JM7586: fim1-mNG::hphR h- | This Study | N/A |
| S. pombe JM7588: arp2-mNG::mNG::hphR h- | This Study | N/A |
| S. pombe JM5198: spn1-mNG::hphR h+ | This Study | N/A |
| S. pombe JM5288: mid1-yomNeonGreen::hphR h+ | This Study | N/A |
| S. pombe JM7587: cdc12-mNG::mNG::hphR h- | This Study | N/A |
| S. pombe JM6907: cdc2-mNeonGreen-hphR lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX h- | This Study | N/A |
| S. pombe JM7860: cdc25-yomNeonGreen::hphR leu1-32:[pJK148-Pwee1-wee1-Twee1] lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:kanMX | This Study | N/A |
| S. pombe JM7881: cdc25-degron-DAmP::kanMX6 cdc13+-internal-sfGFPcp lys3+:ptdh1*:NLS-linker-mTagBFP2:terminatortdh1:natR | This Study | N/A |
| Software and algorithms | ||
| ImageJ 1.53k | NIH Image | https://imagej.nih.gov/ij/ |
| MATLAB | MathWorks | R2021a |
| RStudio | RStudio PBC | 1.4.1717 |
| Intensity_Size_pombe.m | This Study | https://github.com/millerk89/sizer-timer-pombe |
| cell_geometry_dividing_cells.m | This Study | https://github.com/millerk89/sizer-timer-pombe |
| Overlap_analysis_bivariate_data.nb | This Study | https://github.com/millerk89/sizer-timer-pombe |
| overlap_analysis_size.Rmd | This study | https://github.com/millerk89/sizer-timer-pombe |
| model.Rmd | This study | https://github.com/millerk89/sizer-timer-pombe |
| Other | ||
| Cell Asic ONIX, 3.5 – 5.5 μm Y04C-02 | EMD Millipore | N/A |
| Coverglass-bottom dish | MaTek | P35G-1.5-20C |
| Yokogawa CSU-WI spinning disk | Nikon Instruments | N/A |
| 60× 1.4-NA CFI60 Apochromat Lambda S objective lens | Nikon Instruments | N/A |
| 405-, 488-, and 561-nm laser lines | Nikon Instruments | N/A |
| photometrics Prime BSI camera | Nikon Instruments | N/A |
| Eclipse Ti2 | Nikon Instruments | N/A |