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. 2023 Oct 1;14(5):1853–1869. doi: 10.14336/AD.2023.0228

Figure 2.

Figure 2.

CNTN1 overexpression by AAV stereotactic injection in hippocampus triggered cognitive deficits in mice. (A) Schematic illustration of behavior paradigm for assessment of cognitive behavior after AAV-CNTN1virus injection into the dorsal hippocampus in mice. (B) Left panel showed schematic illustration of AAV-Cntn1virus injection into the dorsal hippocampus in mice. The right panel showed Nissl staining of virus injection into the dorsal hippocampus in mice. (C) Quantitative real time qPCR analysis of Cntn1 mRNA expression after AAV-CNTN1 or AAV-Control injection in hippocampus in mice. (D&E) Representative immunoblot (D) and quantitative analysis (E) of CNTN1 expression after AAV-CNTN1 or AAV-Control injection in hippocampus in mice. (F&G) The effect of AAV-CNTN1 or AAV-Control overexpression in hippocampus on Locomotion (F) and anxiety like behaviors (G) in mice as manifested by open field test (OFT) test. (H-K) The cognitive deficits with manifestation by several behavioral traits in different groups, including novel object recognition task (NORT (H)), novel place recognition test (NPRT (I)) and social recognition test (SRT(J&K)). Data were analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison tests except for panel J, which was analyzed by unpaired two-tailed Student’s t test. Data were presented as mean ± sem.*p < 0.05, **p < 0.01 and ***p < 0.001.