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. 2023 Oct 1;14(5):1853–1869. doi: 10.14336/AD.2023.0228

Figure 3.

Figure 3.

CNTN1 overexpression by AAV stereotactic injection activated microglia and astrocyte in the hippocampus. (A-C) Representative images showed immunostaining against microglial marker Iba1 on brain sections in the hippocampus of mice in different groups. (D&E) Quantiative analysis of Iba1 positive microglia of hippocampus in different groups by total number of microglia (D) and integrated optical density (IOD) (E). (F) Quantitative analysis complexity of Iba1 positive microglia in hippocampus with Sholl analysis in different groups. (G) Quantitative real time qPCR detection of mRNA expression levels of CD11b and CD68 in hippocampus in different groups. (H) Quantitative real time qPCR detection of mRNA expression levels of IL1α, IL6, iNOS and CCL2 in hippocampus in different groups. n=4 per group. (I&J) Representative immunoblot (I) and quantitative analysis (J) of vimentin and GFAP expression in hippocampus in different groups. Data in Fig. 3D, E, H, J were analyzed by Kruskal-Wallis statistical test; Data in Fig. 3F was analyzed by two-way ANOVA followed by Bonferroni’s multiple comparison test. Data in Fig. 3G was analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison tests. Data were presented as mean ± sem. *p < 0.05, **p < 0.01 and ****p < 0.0001. Scale bar: 50 μm.