Figure 6.

Microglia inhibition by minocycline ameliorated cognitive deficits in mice with CNTN1 overexpression in hippocampus. (A) Schematic diagram of behavior paradigm of cognitive deficits in mice. (B) Quantitative real time qPCR detection of Cntn1 mRNA expression in hippocampus in different groups. (C&D) Representative immunoblots (C) and quantitative analyses (D) of CNTN1 expression in hippocampus in different groups. (E&F) The total distance traveled (a measurement index of locomotor activity) and anxiety like behaviors in different groups in the OFT test. (G-J) The cognitive deficits with manifestation by several behavioral traits in different groups, including NORT (G), NPRT (H) and SRT (I&J). Data were analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison tests except for Fig. 6I, which was analyzed by unpaired two-tailed Student’s t test. Data were presented as mean ± sem. ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001.