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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: J Comp Neurol. 2023 Jul 21;531(14):1459–1481. doi: 10.1002/cne.25525

Table 1.

Subjects and injection coordinates. Pipette angle = 0° unless otherwise noted. * denotes representative subject used for figure.

Subject Sex Age
(days)		 Injection
site		 Virus Coordinates (mm)
ML, from lambda, unless
otherwise noted
RC, from lambda
DV, from pial surface		 Volume
(nL)		 Titer
(GC/mL)
ID_269953 M 58 OFC AAV1/pENN.AAV.hSyn.TurboRFP.WPRE.RBG (Addgene 105552) −1.5 ML, 8.75 RC, −3.5 DV 90 2.6 x 1014
SUBJ-ID-122 M 365 OFC AAV1/pENN.AAV.hSyn.TurboRFP.WPRE.RBG (Addgene 105552) −1.5 ML, 8.94 RC, −3.2 DV 150 1.5 x 1013
SUBJ-ID-123* M 365 OFC AAV1/pENN.AAV.hSyn.TurboRFP.WPRE.RBG (Addgene 105552) −1.5 ML, 8.95 RC, −3.2 DV 100 1.5 x 1013
SUBJ-ID-294* F 230 OFC AAV1/pENN.AAV.hSyn.TurboRFP.WPRE.RBG (Addgene 105552) −1.2 ML, 9.45 RC, −3.2 DV 80 1.5 x 1013
SUBJ-ID-353* F 271 AuD AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −6.4 ML, 1.05 RC, −0.9 DV 200 1.7 x 1013
−6.4 ML, 1.05 RC, −0.45 DV 200
45° M-L
SUBJ-ID-354 F 271 AuD AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −6.45 ML, 1.05 mm RC, −0.9 DV 200 1.7 x 1013
−6.45 ML, 1.05 mm RC, −0.45 DV 200
45° M-L
SUBJ-ID-355 F 271 AuD AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.25 ML from temporal ridge, 1.34 RC, −0.9 DV 200 1.7 x 1013
−1.25 ML from temporal ridge, 1.34 RC, −0.45 DV 100
45° M-L
SUBJ-ID-56 M 233 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.4 ML from temporal ridge, 3.5 RC, −0.75 DV 200 1 x 1013
−1.4 ML from temporal ridge, 3.5 RC, −0.35 DV 200
45° M-L
SUBJ-ID-110 F 637 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1 ML from temporal ridge, 3.5 RC, −0.9 DV 200 1 x 1013
−1 ML from temporal ridge, 3.5 RC, −0.45 DV 200
45° M-L
SUBJ-ID-111 F 637 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1 ML from temporal ridge, 3.45 RC, −0.9 DV 200 1 x 1013
−1 ML from temporal ridge, 3.45 RC, −0.45 DV 200
45° M-L
SUBJ-ID-121 M 365 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1 ML from temporal ridge, 3.3 RC, −0.75 DV 200 1 x 1013
−1 ML from temporal ridge, 3.3 RC, −0.35 DV 200
45° M-L
SUBJ-ID-133 F 328 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1 ML from temporal ridge, 3.5 RC, −0.75 DV 200 1 x 1013
−1 ML from temporal ridge, 3.5 RC, −0.35 DV 200
SUBJ-ID-297 F 263 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1 ML from temporal ridge, 3.5 RC, −0.75 DV 200 1 x 1013
−1 ML from temporal ridge, 3.5 RC, −0.35 DV 200
SUBJ-ID-298* F 181 Au1 AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.05 ML from temporal ridge, 3.5 RC, −0.9 DV 200 1.7 x 1013
−1.05 ML from temporal ridge, 3.5 RC, −0.45 DV 200
30° M-L
SUBJ-ID-299 F 181 AuV AAVrg-CAG-tdTomato (Addgene 59462) −1.25 ML from temporal ridge, 1.34 RC, −0.9 DV 200 2.5 x 1013
−1.25 ML from temporal ridge, 1.34 RC, −0.45 DV 200
45° M-L
SUBJ-ID-300 F 181 Au1


AuV		 AAVrg/pAAV-hSyn-EGFP (Addgene 50465)



AAVrg-CAG-tdTomato (Addgene 59462)		 −1.25 ML from temporal ridge, 3.55 RC, −0.9 DV 200 1 x 1013
−1.25 ML from temporal ridge, 3.55 RC, −0.45 DV 200
−1.25 ML from temporal ridge, 1.34 RC, −0.9 DV 200 2.5 x 1013
−1.25 ML from temporal ridge, 1.34 RC, −0.45 DV 200
45° M-L
SUBJ-ID-391 M 346 AuV AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −8.0 ML, 1.38 RC, −0.9 DV 200 1.7 x 1013
−8.0 ML, 1.38 RC, −0.45 DV 200
45° M-L
SUBJ-ID-392 M 346 AuV AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −8.0 ML, 1.35 RC, −0.9 DV 200 1.7 x 1013
−8.0 ML, 1.35 RC, −0.45 DV 200
45° M-L
SUBJ-ID-126 F 605 MGV AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −3 ML ,1.5 RC, −4.15 DV 50 1 x 1013
SUBJ-ID-180* M 489 MGV AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −2.85 ML, 1.75 RC, −4.15 DV 80 1 x 1013
SUBJ-ID-181 M 489 MGV AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −2.85 ML, 1.75 RC, −4.18 DV 80 1 x 1013
SUBJ-ID-95 M 408 CIC AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.65 ML, 0.35 RC, −3.65 DV 80 1 x 1013
30° R-C
SUBJ-ID-119 F 396 CIC AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.65 ML, 0.35 RC, −3.65 DV 200 1 x 1013
30° R-C
SUBJ-ID-120 F 396 CIC AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.65 ML, 0.35 RC, −3.65 DV 80 1 x 1013
30° R-C
SUBJ-ID-124* M 396 CIC AAVrg/pAAV-hSyn-EGFP (Addgene 50465) −1.65 ML, 0.35 RC, −3.65 DV 150 1 x 1013
30° R-C
SUBJ-ID-143 F 442 OFC

Au1		 AAV1-hSyn-DIO-mCherry (Addgene 50459)


AAVrg-hSyn-Cre-WPRE.HGH (Addgene 105553)		 −1.5 ML, 9.05 RC, −2.75 DV 100 9 x 1018
1.35 ML from temporal ridge, 3.55 RC, −0.75 DV 200 1.2 x 1013
1.35 ML from temporal ridge, 3.55 RC, −0.35 DV 200
1.35 ML from temporal ridge, 3.7 RC, −0.75 DV 200
1.35 ML from temporal ridge, 3.7 RC, −0.35 DV 200
SUBJ-ID-144 F 442 OFC

Au1		 AAV1-hSyn-DIO-mCherry (Addgene 50459)

AAVrg-hSyn-Cre-WPRE.HGH (Addgene 105553)		 −1.5 ML, 9.05 RC, −2.75 DV 100 9 x 1018
1.35 ML from temporal ridge, 3.2 RC, −0.75 DV 200 1.2 x 1013
1.35 ML from temporal ridge, 3.2 RC, −0.35 DV 200
1.35 ML from temporal ridge, 3.9 RC, −0.75 DV 200
1.35 ML from temporal ridge, 3.9 RC, −0.35 DV 200s