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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Metabolism. 2023 Jun 28;146:155644. doi: 10.1016/j.metabol.2023.155644

Fig. 3. The N-terminal arginylated BiP triggers lipophagy via binding to p62-ZZ domain.

Fig. 3.

(A) Fluorescence of R-BiP-RFP, E-BiP-RFP, V-BIP-RFP, p62 and BODIPY in HepG2 cells upon OA/PA (1 mM/500 μM) loading for 24 h. (B) Schematic of recombinant p62 constructs used. (C) ICC of p62 and BODIPY signals in Hep3B cells expressing p62-full length (p62FL, left panel) or p62-ZZ mutants (p62ΔZZ, right panel), with sequential treatment of OA/PA (1 mM/500 μM, 24 h) and serum starvation (24 h). Scale bar, 10 μm. (D) Scheme of the formation of Nt-arginylated proteins during lipotoxic stress.