Living human pancreas slices were incubated with Lycopersicon esculentum lectin to label the islet microvasculature.
(A) Temporal projection showing changes in islet capillary diameter in a ND donor (nPOD6555) induced by 11 mM glucose.
(B and C) Quantification of capillary diameters in slices from ND (n = 37 capillaries/5 donors), Aab+ (n = 17 capillaries/3 donors), and T1D donors (n = 17 capillaries/5 donors). Each donor is shown with a different symbol.
(C) Mean ± SEM diameter values in 3G and 11G are shown. *p < 0.05 (paired t test).
(D) Quantification of average relative change in capillary diameter (Δdiameter; % baseline) induced by 11G for each group (dots: data point for each donor, bars: mean ± SEM). For each donor, responses from 3–11 vessels were recorded, and an average value was calculated.