KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Neuron-glial antigen 2 (NG2) | R&D | RRID: FAB2585R |
| Platelet-derived growth factor receptor-beta (PDGFRβ) | R&D | RRID: AF385 |
| Alpha smooth muscle actin (αSMA) | Sigma | RRID: A5228 |
| CD31 | Millipore | RRID: 550389 |
| Tyrosine hydroxylase (TH) | Millipore | RRID: AB152 |
| Collagen IV | Abcam | RRID: AB6586 |
| Periostin | R&D | RRID: AF2955 |
| Insulin | Dako | RRID: IR002 |
| Somatostatin | Millipore | RRID: MAB354 |
| CD146 | Abcam | RRID: Ab75769 |
| Alexa Fluor Secondary antibodies | ThermoFischer | |
|
| ||
| Biological samples | ||
|
| ||
| Living human pancreas slices | nPOD | https://npod.org |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Theophylline | Sigma | T1633 |
| Norepinephrine | Tocris | 5169 |
| Endothelin 1 | Sigma | E7764 |
| Angiotensin II | Tocris | 1158 |
| Bosentan | Sigma | 1265 |
| Adenosine | Sigma | A9251 |
| Lycopersicon esculentum Lectin | Vector Labs | DL-1178-1 |
| Fluo-4 | Invitrogen | F14201 |
| Aprotinin | Sigma Aldrich | A6106 |
| Penicillin-Streptomycin-Amphotericin B | Sigma Aldrich | A5955 |
| BrainPhys neuronal medium | Stemcell Technologies | 05790 |
| B27 minus-insulin | Invitrogen | A1895601 |
| Chymostatin | Sigma Aldrich | 11004638001 |
|
| ||
| Deposited data | ||
|
| ||
| Human Pancreas Analysis Program, pancreas single cell RNAseq data repository | HPAP | https://hpap.pmacs.upenn.edu/analysis |
|
| ||
| Software and algorithms | ||
|
| ||
| Prism | GraphPad | Prism9 |
| ImageJ (https://imagej.nih.gov/ij/) | NIH | ImageJ |
| MatLab (www.mathworks.com) | MathWorks | MatLab R2020b |
| Single cell analysis architecture (Github + Zenodo) | JA Lab |
https://github.com/jalmaca/Microvasculature_T1D
https://zenodo.org/record/8122389 |
|
| ||
| Other | ||
|
| ||
| SP5 inverted laser-scanning confocal microscope | Leica | SP5 |
| SP8 upright laser-scanning confocal microscope | Leica | SP8 |