Extended Data Figure 2 |. Cryo-EM data processing for the 7TM domain of RXFP1–G_s.

a, Cryo-EM data processing scheme for the 7TM domain of RXFP1 in complex with G_s. Shown are representative processing steps for one of four individual datasets and the steps used for the combined datasets. b, Representative micrograph from the RXFP1–G_s complex datasets (Scale bar = 50 nm; from 13,457 micrographs). c, Two-dimensional class averages for the 7TM domain of RXFP1 and G proteins. d, Angular distribution of particles in the final refinement for the 7TM domain with G proteins. e, Fourier shell correlation (FSC) used to determine the overall map resolution. f, Map to model FSC curve. g, cryoSPARC non-uniform refinement map colored by local resolution.