Fig. 4:

SiNPs inhibit RANKL induced AP-1 and NF-κB signaling. (a) RAW264.7 cells were treated with RANKL (15ng/ml) in the absence or presence of SiNPs (25μg/ml) for indicated times and whole cell lysate harvested for Western blotting of JNK pathway. (b) Cells were treated with RANKL and SiNPs as in (a) for indicated times and harvested for nuclear lysate and analyzed by Western blotting for AP-1 proteins. Representative of multiple experiments. (c) Cell lysate as in (b) and whole cell lysate was probed for NF-kB pathway proteins. (d) Cells were treated as in (a) for indicated times and harvested for nuclear lysate and analyzed by Western blotting for NF-κB proteins. Representative of multiple experiments.