Table 3.

Sedimentation and diffusion coefficients of minicircle topoisomers with 95% confidence limits

	Sample		(°C)	${\mathrm{s}}_{20,\mathrm{W}}(\mathrm{S})$	${\mathrm{D}}_{20,\mathrm{W}}$ (10−7 cm2 s− 1)
333 bp	Linear		4	6.48	1.44
			12	6.51	1.49
			20	6.57	1.51
			28	6.59	1.57
			37	6.61	1.56
	Nicked		4	7.34	1.64
			12	7.42	1.70
			20	7.47	1.72
			28	7.53	1.76
			37	7.50	1.81
	Supercoiled	Less compact species	4	7.84 ± 0.63	1.61 ± 1.76
			12	7.86 ± 0.39	1.95 ± 0.38
			20	7.99 ± 0.29	2.17 ± 0.73
			28	8.03 ± 0.27	2.40 ± 0.75
			37	7.90 ± 0.41	1.97 ± 0.44
		More compact species	4	8.52 ± 0.30	2.00 ± 1.28
			12	8.52 ± 0.34	1.81 ± 0.90
			20	8.60 ± 0.32	1.85 ± 0.52
			28	8.56 ± 0.39	1.56 ± 0.61
			37	8.65 ± 0.30	1.55 ± 0.29
			(°C)	${\mathrm{s}}_{20,\mathrm{W}}(\mathrm{S})$	${\mathrm{D}}_{20,\mathrm{W}}$ (10−7 cm2 s− 1)
339 bp	Linear		4	6.51	1.46
			12	6.54	1.47
			20	6.61	1.50
			28	6.62	1.54
			37	6.63	1.54
	Nicked		4	7.46	1.62
			12	7.44	1.69
			20	7.50	1.71
			28	7.50	1.73
			37	7.52	1.76
	Supercoiled	Less compact species	4	7.71 ± 0.47	1.63 ± 0.50
			12	8.09 ± 0.34	1.99 ± 0.49
			20	8.10 ± 0.33	2.10 ± 0.51
			28	8.03 ± 0.31	2.16 ± 0.56
			37	7.90 ± 0.39	1.89 ± 0.31
		More compact species	4	8.45 ± 0.32	1.93 ± 0.93
			12	8.57 ± 0.39	1.72 ± 0.42
			20	8.57 ± 0.44	1.71 ± 0.60
			28	8.68 ± 0.44	1.69 ± 0.36
			37	8.68 ± 0.37	1.55 ± 0.34

Where confidence limits are not shown, the confidence limits were exactly zero as assessed by Monte Carlo analysis. The values of linear and nicked samples are obtained from global GA-MC models, whereas the values of supercoiled samples are obtained from global 2DSA-MC models