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. Author manuscript; available in PMC: 2024 Sep 21.
Published in final edited form as: Mol Cell. 2023 Aug 24;83(18):3314–3332.e9. doi: 10.1016/j.molcel.2023.07.029

Figure 3. Hsp104K358D:Y257L:Y662M selectively suppresses α-syn toxicity.

Figure 3.

(A) Δhsp104 yeast integrated with α-syn-YFP (top), FUS (middle), or TDP-43 (bottom) on a galactose-inducible promoter were transformed with Hsp104 variants or an empty vector control. Yeast were spotted onto glucose (uninducing, off) and galactose (inducing, on) media in a five-fold serial dilution. Stars indicate substitution to pore loop in NBD1 (pink), NBD2 (purple). (B) Integrated strains from (A) were induced in the presence of Hsp104 variants or empty vector control for 5 hours (FUS, TDP-43) or 8 hours (α-syn). Yeast were lysed and lysates visualized via Western blot. 3-Phosphoglycerate kinase (PGK) is a loading control. See also Figure S3 and S4.