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. Author manuscript; available in PMC: 2024 Sep 21.
Published in final edited form as: Mol Cell. 2023 Aug 24;83(18):3314–3332.e9. doi: 10.1016/j.molcel.2023.07.029

Key resources table

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit anti-Hsp104 polyclonal Enzo Life Sciences Cat#ADI-SPA-1040-F; RRID:AB_2039208
Rabbit anti-FUS polyclonal Bethyl Laboratories Cat# A300-302A; RRID:AB_309445
Rabbit anti-TDP-43 polyclonal Proteintech Cat#10782; RRID: AB_615042
Rabbit anti-GFP polyclonal Sigma-Aldrich Cat# G1544; RRID: AB_439690
Mouse anti-α-Synuclein monoclonal (Syn303) Kelvin Luk (University of Pennsylvania) RRID:AB_2315395
Mouse anti-PGK1 monoclonal Thermo Fisher Cat#459250; RRID:AB_2532235
IRDye 800CW Goat anti-Mouse IgG secondary antibody LI-COR Cat# 926–32210; RRID:AB_621842
IRDye 680RD Goat anti-Rabbit IgG secondary antibody LI-COR Cat# 926–68071; RRID:AB_10956166
Bacterial and virus strains
Escherichia coli DH5α competent cells Thermo Fisher Cat#18265017
Escherichia coli BL21-CodonPlus (DE3)-RIL competent cells Agilent Cat#230245
Chemicals, peptides, and recombinant proteins
Creatine phosphate Roche Cat#10621722001
Adenosine 5’-triphosphate disodium salt hydrate (ATP) Sigma-Aldrich Cat#A3377
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat#4693159001
Casein fluorescein isothiocyanate from bovine milk (FITC-Casein) Sigma-Aldrich Cat#C0528
Creatine kinase Roche Cat#10127566001
Firefly luciferase Sigma-Aldrich Cat#L9506
Lysozyme Sigma-Aldrich Cat#L6876
His-TEV protease Cupo and Shorter98 N/A
Hsp104 Jackrel et al.34 N/A
Hsp104A503S Jackrel et al.34 N/A
Hsp104K358D This paper N/A
Hsp104K358D:Y257L This paper N/A
Hsp104K358D:Y257T This paper N/A
Hsp104K358D:Y662M This paper N/A
Hsp104K358D:Y257L:Y662M This paper N/A
HAP Jackrel et al.34 N/A
HAPA503S This paper N/A
HAPK358D This paper N/A
HAPK358D:Y257L This paper N/A
HAPK358D:Y257T This paper N/A
HAPK358D:Y662M This paper N/A
HAPK358D:Y257L:Y662M This paper N/A
GroELtrap Jackrel et al.34 N/A
RepA1-25-GFP Lopez et al.99 N/A
ClpP Jackrel et al.34 N/A
Hsc70 Enzo Life Sciences Cat#ADI-SPP-751-F
Hdj2 Enzo Life Sciences Cat#ADI-SPP-405-F
Hsp72 Michalska et al.20 N/A
Hdj1 Michalska et al.20 N/A
α-Syn Jackrel et al.34 N/A
TDP-43 Jackrel et al.34 N/A
Critical commercial assays
ATPase Activity Kit (Colorimetric) Innova Biosciences Cat#601–0120
Luciferase Assay Reagent Promega Cat#E1483
Human α-Synuclein ELISA Kit Thermo Fisher Cat#KHB0061
QuikChange Site-Directed Mutagenesis Kit Agilent Cat# 200518
Deposited data
Raw images of yeast spotting, western blots, and C. elegans experiments Mendeley data doi: 10.17632/t7k9w8txzk.1
Experimental models: Organisms/strains
S. cerevisiae W303a (MATa, can1-100, his3-11, 15, leu2-3, 112, trp1-1, ura3-1, ade2-1) Jackrel et al.34 N/A
S. cerevisiae W303a∆hsp104 (MATa, can1-100, his3-11, 15, leu2-3, 112, trp1-1, ura3-1, ade2-1, hsp104::KanMX) Jackrel et al.34 N/A
S. cerevisiae W303aΔhsp104-pAG303GAL-α-syn-YFP-pAG304GAL-α-syn-YFP Jackrel et al.34 N/A
S. cerevisiae W303aΔhsp104-pAG303GAL-FUS Jackrel et al.34 N/A
S. cerevisiae W303aΔhsp104-pAG303GAL-TDP-43 Jackrel et al.34 N/A
S. cerevisiae W303aΔhsp104-pAG303GAL-TDP-43-GFPS11-pAG305GAL-GFPS1-10 Jackrel et al.34 N/A
S. cerevisiae W303aΔhsp104-pAG303GAL-FUS-GFP Jackrel et al.34 N/A
C. elegans UA44 (baln11 [Pdat-1::α-syn, Pdat-1: :GFP]) Cao et al.85 N/A
C. elegans UA367 (baEx198 a,b,c [Pdat-1::HSP104-K358D, rol-6];baln11 [Pdat-1:: α-syn, Pdat-1 ::GFP]) This study N/A
C. elegans UA368 (baEx199 a,b,c [Pdat-1::HSP104-K358D:Y257L, rol-6];baln11 [Pdat-1:: α-syn, Pdat-1 ::GFP]) This study N/A
C. elegans UA369 (baEx200 a,b,c [Pdat-1::HSP104-K358D:Y257T, rol-6];baln11 [Pdat-1::α-syn, Pdat-1 ::GFP]) This study N/A
C. elegans UA370 (baEx201 a,b,c [Pdat-1::HSP104-K358D:Y257L:Y662M, rol-6];baln11 [Pdat-1:: α-syn, Pdat-1::GFP]) This study N/A
C. elegans UA371 (baEx202 a,b,c [Pdat-1::HSP104-K358D: Y662M, rol-6];baln11 [Pdat-1:: α-syn, Pdat-1 ::GFP]) This study N/A
C. elegans UA256 (baEx147 a,b,c [Pdat-1::Hsp104 WT, Pmyo-2::mCherry]; baIn11 [Pdat-1:: α-syn, Pdat-1::GFP]) Jackrel et al.34 N/A
C. elegans UA259 (baEx150 a,b,c [Pdat-1::Hsp104-A503S, Pmyo-2::mCherry]; baIn11 [Pdat-1::α-syn, Pdat-1::GFP]) Jackrel et al.34 N/A
C. elegans UA262 (baEx153 a,b,c [Pdat-1::Hsp104-DPLF:A503V, Pmyo-2::mCherry]; baIn11[Pdat-1::α-syn, Pdat-1::GFP]) Jackrel et al.34 N/A
Oligonucleotides
See Table S1
Recombinant DNA
pRS416GAL Jackrel et al.34 N/A
pRS416GAL-Hsp104 Jackrel et al.34 N/A
pRS416GAL-Hsp104A503S Jackrel et al.34 N/A
pRS416GAL-Hsp104A503S:Y257L This study N/A
pRS416GAL-Hsp104A503S:Y257T This study N/A
pRS416GAL-Hsp104A503S:Y662M This study N/A
pRS416GAL-Hsp104A503S:Y257L:Y662M This study N/A
pRS416GAL-Hsp104K358D This study N/A
pRS416GAL-Hsp104K358D:Y257L This study N/A
pRS416GAL-Hsp104K358D:Y257F This study N/A
pRS416GAL-Hsp104K358D:Y257W This study N/A
pRS416GAL-Hsp104K358D:Y257I This study N/A
pRS416GAL-Hsp104K358D:Y257V This study N/A
pRS416GAL-Hsp104K358D:Y257M This study N/A
pRS416GAL-Hsp104K358D:Y662L This study N/A
pRS416GAL-Hsp104K358D:Y662F This study N/A
pRS416GAL-Hsp104K358D:Y662W This study N/A
pRS416GAL-Hsp104K358D:Y662I This study N/A
pRS416GAL-Hsp104K358D:Y662V This study N/A
pRS416GAL-Hsp104K358D:Y662M This study N/A
pRS416GAL-Hsp104D484K This study N/A
pRS416GAL-Hsp104D484K:Y257L This study N/A
pRS416GAL-Hsp104D484K:Y257F This study N/A
pRS416GAL-Hsp104D484K:Y257W This study N/A
pRS416GAL-Hsp104D484K:Y257I This study N/A
pRS416GAL-Hsp104D484K:Y257V This study N/A
pRS416GAL-Hsp104D484K:Y257M This study N/A
pRS416GAL-Hsp104D484K:Y662L This study N/A
pRS416GAL-Hsp104D484K:Y662F This study N/A
pRS416GAL-Hsp104D484K:Y662W This study N/A
pRS416GAL-Hsp104D484K:Y662I This study N/A
pRS416GAL-Hsp104D484K:Y662V This study N/A
pRS416GAL-Hsp104D484K:Y662M This study N/A
pRS416GAL-Hsp104Y257L This study N/A
pRS416GAL-Hsp104Y662L This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662L This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662F This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662W This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662M This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662S This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662T This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662Q This study N/A
pRS416GAL-Hsp104K358D:Y257L:Y662N This study N/A
pRS416GAL-Hsp104K218T:K620T Torrente et al.39 N/A
pRS416GAL-Hsp104K218T:A503S:K620T This study N/A
pRS416GAL-Hsp104K218T:K358D:K620T This study N/A
pRS416GAL-Hsp104K218T:Y257L:K358D:K620T This study N/A
pRS416GAL-Hsp104K218T:Y257T:K358D:K620T This study N/A
pRS416GAL-Hsp104K218T:K358D:K620T:Y662M This study N/A
pRS416GAL-Hsp104K218T:Y257L:K358D:K620T:Y662M This study N/A
pRS416GAL-Hsp104K358D:Y257S This study N/A
pRS416GAL-Hsp104K358D:Y257T This study N/A
pRS416GAL-Hsp104K358D:Y257Q This study N/A
pRS416GAL-Hsp104K358D:Y257N This study N/A
pRS416GAL-mCherry This study N/A
pRS416GAL-Hsp104-mCherry This study N/A
pRS416GAL-Hsp104K358D-mCherry This study N/A
pRS416GAL-Hsp104A503S-mCherry This study N/A
pRS416GAL-Hsp104K358D:Y257T-mCherry This study N/A
pRS416GAL-Hsp104K358D:Y257L-mCherry This study N/A
pRS416HSE-Hsp104 Jackrel et al.34 N/A
pRS416HSE-Hsp104A503V Jackrel et al.34 N/A
pRS416HSE-Hsp104K358D This study N/A
pRS416HSE-Hsp104K358D:Y257L This study N/A
pRS416HSE-Hsp104K358D:Y257T This study N/A
pRS416HSE-Hsp104K358D:Y662M This study N/A
pRS416HSE-Hsp104K358D:Y257L:Y622M This study N/A
pAG413GAL Alberti et al.100 N/A
pAG413GAL-α-syn This study N/A
pAG413GAL-α-synE46K This study N/A
pAG413GAL-α-synA53T This study N/A
pAG413GAL-α-syn1-95 This study N/A
pAG413GAL-α-syn1-115 This study N/A
pAG413GAL-α-syn1-125 This study N/A
pAG415GAL Alberti et al.100 N/A
pAG415GAL-α-syn This study N/A
pAG415GAL-α-synE46K This study N/A
pAG415GAL-α-synA53T This study N/A
pAG415GAL-α-syn1-95 This study N/A
pAG415GAL-α-syn1-115 This study N/A
pAG415GAL-α-syn1-125 This study N/A
pNOTAG-Hsp104 Jackrel et al.34 N/A
pNOTAG-Hsp104A503S Jackrel et al.34 N/A
pNOTAG-Hsp104K358D This study N/A
pNOTAG-Hsp104K358D:Y257L This study N/A
pNOTAG-Hsp104K358D:Y257T This study N/A
pNOTAG-Hsp104K358D:Y662M This study N/A
pNOTAG-Hsp104K358D:Y257L:Y662M This study N/A
pNOTAG-HAP Jackrel et al.34 N/A
pNOTAG-HAPA503S This study N/A
pNOTAG-HAPK358D This study N/A
pNOTAG-HAPK358D:Y257L This study N/A
pNOTAG-HAPK358D:Y257T This study N/A
pNOTAG-HAPK358D:Y662M This study N/A
pNOTAG-HAPK358D:Y257L:Y662M This study N/A
pTrc99A-GroELtrap Jackrel et al.34 N/A
pBAD-RepA1-25-GFP Lopez et al.99 N/A
pET28b-ClpP-his Jackrel et al.34 N/A
pE-SUMO-Hsp72 This study N/A
pE-SUMO-Hdj1 Michalska et al.20 N/A
pHis-TEV Cupo and Shorter98 N/A
pT7-7 α-syn Lo Bianco et al.27 N/A
pDUET-GST-TEV-TDP-43 Johnson et al.71 N/A
Software and algorithms
Prism 9 GraphPad N/A
ImageJ Rueden et al.101 N/A
Other
Custom code for a semi-automated procedure was used to quantify colocalization between the red and green images. Zenodo doi: 10.5281/zenodo.8185164