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. Author manuscript; available in PMC: 2024 Sep 15.
Published in final edited form as: J Immunol. 2023 Sep 15;211(6):1042–1051. doi: 10.4049/jimmunol.2300361

Fig. 4. UrIg is expressed in the liver and kidney.

Fig. 4

cDNA generated from nurse shark “G” total RNA was used for PCR amplification of UrIg. Primers were casted at the 5’ of the C1 and 3’ of the C3 domain to amplify the 792bp constant regions (arrow marked with C1-C3). These primers also amplify the C2-less short, spliced form (C2-less) expressed at very low levels. The uniform band at the bottom of the gel is presumably primer dimers which also appear in the control (NTC: non template control) lane. NDPK was used as a PCR control since it is nearly ubiquitously expressed. Size marker: phiX174 HaeIII.