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. Author manuscript; available in PMC: 2024 Jul 31.
Published in final edited form as: Vaccine. 2023 Jul 6;41(34):4996–5002. doi: 10.1016/j.vaccine.2023.06.081

Figure 3. 12ISP and 19ISP have similar effects on tick feeding and erythema at the tick bite side.

Figure 3.

Guinea pigs were immunized three times with 12ISP (n=5), 19ISP (n=5) or control Luc mRNA-LNP (n=5) and each challenged with 30 I. scapularis nymphs two weeks after the last immunization. The animals were monitored to assess erythema, tick detachment, recovery, and engorgement. The graph shows the percentage of ticks that remain attached and the detachment at a given time point (A). The success of tick feeding was determined by examining engorgement weights of the recovered ticks (B). Erythema at each tick bite site was calculated as the percent of nymphs (30 per animal as 100%) showing redness on each animal (C). Animals immunized with 19ISP and 12ISP were compared with each other and statistical significance was determined by Mann Whitney test, P value = 0.0079 (A), P value <0.0001 (B); (C) two-way ANOVA; P value < 0.0001. The error bars represent mean with SD.