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. Author manuscript; available in PMC: 2024 Jul 31.
Published in final edited form as: Vaccine. 2023 Jul 6;41(34):4996–5002. doi: 10.1016/j.vaccine.2023.06.081

Figure 5. Screen of candidate mRNA-LNP cocktails: Tick feeding kinetics on immunized guinea pigs with different combinations of mRNA-LNPs.

Figure 5.

Guinea pigs were immunized three times with six different combinations of mRNA-LNPs and challenged with I. scapularis nymphs. Evidence of tick rejection and the tick feeding kinetics were monitored for the duration of the experiment and the graph shows the percentage of ticks that remain attached and the detachment at a given time point (A). The success of tick feeding was determined by examining engorgement weights of the recovered ticks (B). Erythema at each tick bite site was calculated as the percent of nymphs (30 per animal) showing redness on each animal (C). One animal (n=1) was used for each mRNA-LNP combination plus Luc control animal, totaling 7 animals. The images show erythema on the backs of animals representing 7ISP, 8ISP, 12ISP or control animals at the indicated time point (D).