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. Author manuscript; available in PMC: 2024 Jul 31.
Published in final edited form as: Vaccine. 2023 Jul 6;41(34):4996–5002. doi: 10.1016/j.vaccine.2023.06.081

Figure 6. 12ISP and 19ISP mRNA-LNP immunizations elicit antibody responses to specific I. scapularis antigens.

Figure 6.

Two weeks after the last immunization, sera from 19ISP (n=5) and 12 ISP (n=5) animals were collected to assess the humoral immune response. The serum sample binding to specific recombinant proteins corresponding to the mRNAs in each group. IgG antibodies were detected by ELISA with basis of optical density (OD) 450 nm. Sera were diluted 1:500, 1:5000, 1:50,000 and control sample at 1:500. The animals immunized with 12ISP (A) had detectable antibodies against all recombinant proteins used (Salp14, IsPDI3, TIX5, P32, Salp26A, SG27, TSLPI, Salp15, Salp25D, SG10 and P11) and 19ISP (B) for 10 proteins (Salp14, Salp15, Salp25D, Salp26A, TSLPI, IsPDIA3, TIX, P32, SG10 and SG27). Data are represented as mean ± SEM.