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. Author manuscript; available in PMC: 2023 Sep 27.
Published in final edited form as: Cell Rep. 2023 Jul 16;42(7):112818. doi: 10.1016/j.celrep.2023.112818

Figure 4. MMP9 manipulations affect metaphocyte migration speed and airineme extension frequency.

Figure 4.

(A) Differential expression of MMP9 in ameboid (arrowhead) and dendritic macrophage subpopulation (arrow).

(B) Treatment with an MMP9 inhibitor significantly reduced the migration speed of the ameboid macrophage subpopulation, while dendritic macrophages were unaffected (n = 51, 64, 45, and 68 cells), four trunks each. *The n numbers represent the experimental groups from left to right order for all the following figure legends.

(C) Consistently, the migration speed of grn2+ metaphocytes was significantly decreased in MMP9 inhibitor-treated fish (n = 28 and 31 cells; 4 trunks each).

(D) Airineme extension speed reduction in the presence of MMP9 inhibitor (n = 53 and 23; 3 trunks each).

(E) Xanthoblasts in MMP9 inhibitor treated fish had fewer airinemes than controls (n = 10 and 16 time-lapse positions; 4 and 5 trunks).

(F) Localization of ameboid macrophages were shifted more apically upon MMP9 inhibitor treatment but no effects on dendritic macrophage subpopulation (n = 31, 45, 80, and 75 cells; 3, 3, 4, and 5 trunks).

(G) Xanthoblasts in mmp9+ cell-depleted fish had fewer airinemes than controls (n = 9 and 9 time-lapse positions; 3 trunk each).

(H) Xanthoblasts in mmp9 overexpressed fish extend more airinemes than controls (n = 9 and 12 time-lapse positions; 4 trunk each).

(I) The migration speed of the ameboid macrophage subpopulation was increased, while dendritic macrophages remained unaffected (n = 15, 17, 15, and 24 cells; 3 trunk each). Scale bar, 20 μm (A). Statistical significances were assessed by Student t test. Bars display mean, and error bars display SEM.