KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMSO | Sigma-Aldrich | D8418 |
| metronidazole | Sigma-Aldrich | M3761 |
| MMP9 inhibitor II | Calbiochem | 44–429-310MG |
| Critical Commercial Assays | ||
| LR Clonase II | Invitrogen | 12538120 |
| Gibson Assembly®Master Mix | NEB | E2611L |
| SuperScript™ III Reverse Transcriptase | Invitrogen | 18080093 |
| Experimental Models: Organisms/Strains | ||
| Zebrafish: ABb wild-type | Parichy lab | N/A |
| Zebraf ish: Tg(cldn3c:GFP)hkz021tTg | Wen lab | ZFIN: ZDB-ALT-191122–2 |
| Zebrafish: Tg(mpeg1:loxp-DsRedxloxp-EGFP)hkz015tTg | Wen lab | ZFIN:ZDB-ALT-161018–7 |
| Zebrafish: TgBAC(grn2:EGFP-NTR)hkz034Tg | Wen lab | ZFIN:ZDB-ALT-210708–2 |
| Zebrafish: Tg(tyrp 1b:PALMmCherry)wprt11Tg | Parichy lab | ZDB-ALT-141218–2 |
| Zebrafish:Tg(mpeg1.1:LOX2272-LOXP-dTomato-LOX2272-Cerulean-LOXP-EYFP)w201Tg | Ramakrishnan Lab | ZDB-ALT-150512–3 |
| Zebrafish: TgBAC(mmp9:EGFP)tyt206Tg | Kawakami Lab | ZDB-ALT-180501–1 |
| Zebrafish: TgBAC(mmp9:EGFP-NTR)tyt207Tg | Kawakami Lab | ZDB-ALT-180501–2 |
| Zebrafish: Tg(mpeg1:mmp9-v2a-nVenus)irrt15Tg | This paper | N/A |
| Zebrafish: Tg(krt4:PALMegfp)irrt1Tg | This paper | N/A |
| Zebrafish: Tg(krtt1c19e:LY-Tomato)sq16Tg | Carney lab | ZDB-ALT-140424–2 |
| Oligonucleotides | ||
| aox5_forward, 5′-CAGAGTGACGTCTGGTCTTACG-3′ | This paper | N/A |
| aox5_reverse, 5′-GGACATCTGATAGCCACACTTG-3′ | This paper | N/A |
| pmela_forward, 5′-CICGGAGIICIGIIIIICGIII-3′ | This paper | N/A |
| pmela_reverse, 5′-AAGGTACTGCGCTTATTCCTGA-3′ | This paper | N/A |
| actin_forward, 5′-CTTGCTCCTTCCACCATGAA-3′ | This paper | N/A |
| actin_reverse, 5′-CTGCTTGCTGATCCACATCT-3′ | This paper | N/A |
| mmp9_forward, 5′-CATTAAAGATGCCCTGATGTATCCC-3′ | This paper | N/A |
| mmp9_reverse, 5′-AGTGGTGGTCCGTGGTTGAG-3′ | This paper | N/A |
| Recombinant DNA | ||
| cldnh:mCherry | This paper | N/A |