PCR of the 16S-23S rRNA ISR. Lanes contain B. abortus (lane 1, 30101; lane 2, 30102), B. canis (lane 3, 30201; lane 4, 30202), B. melitensis (lane 5, 30401; lane 6, ATCC 31242), B. suis (lane 7, 30301; lane 8, 4312), and E. coli (lane 9, K-12). Note that the four species of Brucella have identical profiles which are distinct from that of E. coli. Numbers to the left indicate standard (STD) pGEM base pair markers.