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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Biomaterials. 2023 Jul 13;301:122245. doi: 10.1016/j.biomaterials.2023.122245

Figure 8. DOT1L silencing reduces AURKB and AURKB inhibition mitigates IH in injured rat carotid arteries.

Figure 8.

To induce IH, balloon injury of common carotid artery was performed in Sprague-Dawley rats. Immediately after the injury, lentivirus was intra-luminally infused into the injured artery wall to express scrambled or DOT1L-specific shRNA. In a separate experiment, the AURKB-selective inhibitor barasertib (10 mg/kg) was applied in perivascular hydrogel. Arteries were collected 14 days after injury for immunostaining or morphometric analysis. N, neointima (between arrows); M, media; A, adventitia. Scale bar:100 μm.

A. DOT1L silencing reduces AURKB. Quantification: Mean ± SEM, n=4 rats; unpaired Student’s t-test: *p<0.05.

B-C. AURKB inhibition mitigates IH (neointima vs media area ratio). Quantification: Mean ± SEM, n= 6 rats; unpaired Student’s t-test: *p<0.05.