Table 1. ΔLD100 in inflammasome-deficient mice.
The calculation of ΔLD100 requires publications that investigate WT and inflammasome-deficient mice and utilize multiple doses within a single study155. In cases where only a single dose is administered, we rely on estimations based on studies conducted by the Re lab. Specifically, during Burkholderia pseudomallei infection, the Re lab demonstrated that 100% of WT mice survived a very low dose challenge, whereas 0% of inflammasome-deficient mice survived. Notably, when the Re lab increased the dose from 25 CFU to 200 CFU, 0% of WT mice survived. This data indicates an 8-fold change in the lethal dose. Therefore, if a single dose is used and all WT mice survive while all inflammasome-deficient mice succumb, it can only be interpreted as an eight-fold or greater change in the lethal dose. Notably, most published infection studies utilize only a single infectious dose8,9. Consequently, the full extent of the effect of inflammasomes in preventing lethal infection remains to be fully quantitated.