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(a) Score plot of the OPLS-DA model built with the 1H NMR spectra of urine samples collected at time T1: controls (blue circle); NDM-diet (green circle); NDM-insulin (red circle). (b) Permutation plot from 400 permutation tests with Q2 intercept of −0.298. The horizontal axis shows the correlation between the permuted and actual data, while the vertical axis displays the cumulative values of R2 and Q2. The intercept gives an estimate of the overfitting phenomenon.
