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. 2020 Jun 26;2020:9350905. doi: 10.34133/2020/9350905

Table 1.

Different types of plant prime editor (PPE), their features, and editing efficiency in different target sites.

Target gene PPE type PPE features (PBSa length (nt), RTb template length (nt), and RT type) Mutation type Editing efficiencyi Refs
Desired Undesired
BFP PPE3b 14, 12, M-MLVc 2 bp Subsf 4.40% NRj [2]
BFP PPE3b 14, 12, CaMVd 2 bp Subs 3.70% NR [2]
BFP PPE3b 14, 12, Retrone 2 bp Subs 2.40% NR [2]
OsCDC48 PPE2 12, 9, M-MLV 6 bp Delg 8.20% 4.6% [2]
OsCDC48 PPE3 12, 9, M-MLV 6 bp Del 21.80% NR [2]
OsCDC48 PPE3 12, 15, M-MLV 3 bp Subs 2.60% NR [2]
OsCDC48 PPE3b 12, 9, M-MLV 6 bp Del 11% NR [2]
OsCDC48 PPE3b 12, 9, CaMV 6 bp Del 5.80% NR [2]
OsCDC48 PPE3 10, 17, M-MLV 1 bp Subs 14.30% NR [2]
OsCDC48 PPE2 12, 13, M-MLV 3 bp Insh 1.98% 0.7% [2]
OsCDC48 PPE2 12, 18, M-MLV 1 bp Subs 5.70% 3.8% [2]
OsCDC48 PPE3b 12, 13, M-MLV 3 bp Ins 1.88 0.03% [2]
OsCDC48 PPE3b 12, 18, M-MLV 1 bp Subs 3.0% 2.0% [2]
OsCDC48 PPE3b 12, 18, CaMV 1 bp Subs 0.30 NR [2]
OsCDC48 PPE2 12, 15, M-MLV 3 bp Del ~0.05% ~0.05% [2]
OsCDC48 PPE3b 12, 15, M-MLV 3 bp Del ~0.05% ~0.05% [2]
OsALS PPE2 10-12, 16-17, M-MLV 1 bp Subs 0.28% 0.035% [2]
OsALS PPE3 12-13, 13-16, M-MLV 1 bp Subs 0.35% 0.52% [2]
OsDEP1 PPE2 13, 13, M-MLV 1 bp Subs 0.10-0.3% 0.03-0.3% [2]
OsDEP1 PPE3 13, 11-13, M-MLV 1 bp Subs 0.10-0.3% 0.1-0.2% [2]
OsEPSPS PPE2 13, 11-20, M-MLV 1 bp Subs 0.80-1% 0.3-0.6% [2]
OsEPSPS PPE3 13, 20, M-MLV 1 bp Subs 2.27% 2.66% [2]
OsEPSPS PPE3 13, 17, M-MLV 1 bp Subs 1.55% 1.53% [2]
OsEPSPS PPE2 13, 17, M-MLV 1 bp Subs 0.10 0.2 [2]
OsEPSPS PPE3 13, 17, M-MLV 1 bp Subs 0.10 0.2 [2]
OsLDMAR PPE2 12, 15, M-MLV 1 bp Subs 0.35% 0.1% [2]
OsLDMAR PPE3 12, 15, M-MLV 1 bp Subs 0.73% 0.1% [2]
OsGAPDH PPE2 12, 16, M-MLV 1 bp Subs 1.40% 0.16% [2]
OsGAPDH PPE3 12, 16, M-MLV 1 bp Subs 1.60% 0.24% [2]
OsAAT PPE2 12, 13, M-MLV 2 bp Subs 0.12% NR [2]
OsAAT PPE2-R 12, 13, M-MLV 2 bp Subs 0.04% NR [2]
OsAAT PPE3b 12, 13, M-MLV 2 bp Subs 0.20% NR [2]
OsAAT PPE3b-R 12, 13, M-MLV 2 bp Subs 0.45% NR [2]
TaUbi10- PPE2 13, 16, M-MLV 1 bp Subs 0.06% 0.13% [2]
TaUbi10 PPE3 13, 16, M-MLV 1 bp Subs 0.20% 0.1% [2]
TaUbi10 PPE2 12, 12, M-MLV 1 bp Subs 0.40-0.80% 0.1-0.2% [2]
TaGW2 PPE2 11, 11, M-MLV 1 bp Subs 0.30% 0.03% [2]
TaGW2 PPE3 11, 11, M-MLV 1 bp Subs 0.36% 0.12% [2]
TaGASR7 PPE2 12, 18, M-MLV 1 bp Subs 1.40% 0.00% [2]
TaGASR7 PPE3 12, 18, M-MLV 1 bp Subs 0.67% 0.00% [2]
TaLOX2 PPE2 12, 14, M-MLV 1 bp Subs 0.30% 0.068% [2]
TaLOX2 PPE3 12, 14, M-MLV 1 bp Subs 0.22% 0.05% [2]
TaMLO PPE2 12, 12, M-MLV 1 bp Subs 0.60% 0.00% [2]
TaMLO PPE3 12, 12, M-MLV 1 bp Subs 0.40% 0.00% [2]
TaDME1 PPE2 13, 14, M-MLV 1 bp Subs 1.30% 0.07% [2]
TaDME1 PPE3 13, 14, M-MLV 1 bp Subs 1.00% 1.0% [2]
HPTII PPE3-t 13, 28, M-MLV 3 bp Subs 9.38% NR [26]
OsALS PPE2-WT 13, 13, M-MLV 1 bp Subs 0.05% NR [27]
OsALS PPE2-V01 13, 13, M-MLV 1 bp Subs 0.10% NR [27]
OsALS PPE3b-V01 13, 13, M-MLV 1 bp Subs 0.10% NR [27]
OsKO2 PPE2-V01 13, 19, M-MLV 1 bp Subs 0.13% NR [27]
OsDEP1 PPE2-WT 13, 13, M-MLV 1 bp Subs 0.01% NR [27]
OsDEP1 PPE2-V01 13, 13, M-MLV 1 bp Subs 0.15% NR [27]
OsDEP1 PPE3-V02 10, 22, M-MLV 1 bp Subs 0.03% NR [27]
OsDEP1 PPE3-V02 12, 19, M-MLV 1 bp Subs 0.23% NR [27]
OsDEP1 PPE3-V02 13, 13, M-MLV 1 bp Subs 0.67% NR [27]
OsDEP1 PPE3-V02 14, 17, M-MLV 1 bp Subs 0.35% NR [27]
OsDEP1 PPE3-V02 12, 11, M-MLV 3 bp Ins 0.90% NR [27]
OsDEP1 PPE3-V02 13, 17, M-MLV 3 bp Ins 0.50% NR [27]
OsDEP1 PPE3-V02 14, 25, M-MLV 3 bp Ins 0.075% NR [27]
OsDEP1 PPE3-V02 16, 14, M-MLV 3 bp Ins 1.53% NR [27]
OsPDS PPE2-V01 13, 13, M-MLV 1 bp Subs 0.06% NR [27]
OsPDS PPE3b-V02 10-16, 10-25, M-MLV 3 bp Ins 0.03-0.25% NR [27]
OsPDS PPE2-V01 10-16, 10-25, M-MLV 3 bp Ins 0.05-0.86% NR [27]
OsPDS PPE3-V02 10-16, 10-19, M-MLV 3 bp Ins 0.08-0.8% NR [27]
OsEPSPS PPE3b-V01 13, 23, M-MLV 1 bp Subs 0.36% NR [27]
OsEPSPS PPE3b-V01 13, 18, M-MLV 1 bp Subs 0.13% NR [27]
OsGRF4 PPE3b-V01 13, 15, M-MLV 1 bp Subs 0.16% NR [27]
GFP, ALS, APO1 Sp-PE2 13, 13, M-MLV 1 bp Subs 0-17.1% NR [28]
OsSLR1 Sp-PE3 13, 13, M-MLV 3 bp Del 0.00% NR [28]
OsSPL14, APO2 Sp-PE3 13, 13, M-MLV 24 bp Ins 0.00% NR [28]
GFP, ALS, HPT Sa-PE3 13, 16-34, M-MLV 1 bp Subs 0-32.65% NR [28]
OsPDS pPE2 13, 12, M-MLV 1 bp Ins 7.30% NR [29]
OsPDS pPE2 13, 13, M-MLV 2 bp Ins 12.5% NR [29]
OsPDS pPE2 13, 14, M-MLV 3 bp Ins 19.8% NR [29]
OsPDS pPE2 13, 11, M-MLV 28 bp Del 0.00% NR [29]
OsPDS pPE2 13, 11, M-MLV 1 bp Subs 0-31.25% NR [29]
OsACC pPE2 10-15, 10-34, M-MLV 1 bp Subs 0-14.6% NR [29]
OsACC pPE3 13, 10, M-MLV 1 bp Subs 10.4-18.75% NR [29]
OsACC pPE3b 13, 10, M-MLV 1 bp Subs 6.25% NR [29]
OsWX1 pPE2 15, 31, M-MLV 1 bp Subs 7.30% NR [29]

aPBS: primer binding site; bRT: reverse transcriptase; cM-MLV: Moloney murine leukemia virus; dCaMV: Cauliflower mosaic virus; eRetron: retron-derived RT (RT-retron) from E. coli BL21; fSubs: substitution; gDel: deletion; hIns: insertion; iData obtained from the published graph using the WebPlotDigitizer software (https://apps.automeris.io/wpd/); jNR: not reported.