Figure 1.

Immature CiN cells are not yet neuronal-like cells. (A) A schematic representation of the induction protocol for immature CiN cells. (B) Control cells cultured for 8 d without 7 chemical compounds were stained with anti-Tuj1 (green) and anti-MAP2 (red) antibodies, which are neural cell markers. (C) Immature CiN cells were cultured for 8 d with 7 chemical compounds and stained with anti-Tuj1 and anti-MAP2 antibodies, which are neural cell markers. Nuclei were stained with DAPI (blue): scale bar, 200 μm.