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. 2023 Sep 11;24(18):13949. doi: 10.3390/ijms241813949

Figure 2.

Figure 2

Effects of piperine on induction of apoptosis in HSC-3 cells. (A,B) Fluorescence microscopic images of HSC-3 cells treated with piperine (100 and 150 µM for 24 h) and stained with DAPI. The arrows indicate DAPI-positive HSC-3 cell. (C,D) FACS results of HSC-3 cells treated with piperine (100 and 150 µM for 24 h) and were double stained with annexin-V/PI. (E,F) Expression levels of PARP, Bax and Bcl-2 in HSC-3 cells after piperine (100 and 150 µM for 24 h) treatment. β-actin was used as loading control, and the quantification was performed using ImageJ. Data are presented as mean and SD for three samples. * p < 0.05, compared with the control group.