TABLE 1.
Synthetic oligonucleotides used in PCR and hybridization analyses
| Primer or probea | Nucleotide sequence (5′ to 3′) | Chemistry and source |
|---|---|---|
| ITS3 | GCA TCG ATG AAG AAC GCA GC | 5.8S rDNA universal 5′ primer |
| ITS4 | TCC TCC GCT TAT TGA TAT GC | 28S rDNA universal 3′ primer |
| BP | CA TGC CTG TTT GAG CGT C(GA)T T | 5′-end-labeled biotin probe; generic 5.8S rDNA probe |
| CA | AT TGC TTG CGG CGG TAA CGT CC | 5′-end-labeled digoxigenin probe; ITS2 region of C. albicans |
| DB | AA GGC GGT CTC TGG CGT CGC CC | 5′-end-labeled digoxigenin probe; ITS2 region of C. dubliniensis |
| CG | TA GGT TTT ACC AAC TCG GTG TT | 5′-end-labeled digoxigenin probe; ITS2 region of C. glabrata |
| CGE | TT TAC CAA CTC GGT GTT GAT CT | 5′-end-labeled digoxigenin probe; ITS2 region of C. glabrata |
| GU | CC CGG CCT TAC AAC AAC CAA AC | 5′-end-labeled digoxigenin probe; ITS2 region of C. guilliermondii |
| CH | CC GTT GGT GGA TTT GTT TCT AA | 5′-end-labeled digoxigenin probe; ITS2 region of C. haemulonii |
| KF | GA GAC TCA TAG GTG TCA TAA AG | 5′-end-labeled digoxigenin probe; ITS2 region of C. kefyr |
| CK | GG CCC GAG CGA ACT AGA CTT TT | 5′-end-labeled digoxigenin probe; ITS2 region of C. krusei |
| LA2 | AA AGC GAG GGG CCT TCT GCG CG | 5′-end-labeled digoxigenin probe; ITS2 region of C. lambica |
| LA4 | GC GAG GGG CCT TCT GCG CGA AC | 5′-end-labeled digoxigenin probe; ITS2 region of C. lambica |
| LU | CT CCG AAA TAT CAA CCG CGC TG | 5′-end-labeled digoxigenin probe; ITS2 region of C. lusitaniae |
| NS | AC TGA GCG AAG TAC ACA ACA CT | 5′-end-labeled digoxigenin probe; ITS2 region of C. norvegensis |
| NC | AC GAG CGT CTG CTG GCT CCA CA | 5′-end-labeled digoxigenin probe; ITS2 region of C. norvegica |
| CP | AC AAA CTC CAA AAC TTC TTC CA | 5′-end-labeled digoxigenin probe; ITS2 region of C. parapsilosis |
| PL | AT CAG CTA GGC AGG TTT AGA AG | 5′-end-labeled digoxigenin probe; ITS2 region of C. pelliculosa |
| CR | AG TTA AGC TTG TTA CAG ACT CA | 5′-end-labeled digoxigenin probe; ITS2 region of C. rugosa |
| CT | AA CGC TTA TTT TGC TAG TGG CC | 5′-end-labeled digoxigenin probe; ITS2 region of C. tropicalis |
| CU2 | AC TCG TTA TTT TCC AGA CAG AC | 5′-end-labeled digoxigenin probe; ITS2 region of C. utilis |
| VS | CT ACC AAA ACG CTT GTG CAG TC | 5′-end-labeled digoxigenin probe; ITS2 region of C. viswanathii |
| CZ | TC GTT GAC CAG TAT AGT ATT TG | 5′-end-labeled digoxigenin probe; ITS2 region of C. zeylanoides |
a
Patents have been issued or are pending for all Candida species probes.