Figure 1.

Endothelial cells, but not stromal cells, enabled VM formation by TNBC cells. EA.hy926 endothelial cells (ECs), but not N30 stromal cells (SCs), promoted the formation of vasculogenic mimicry by two different TNBC cell lines (HCC1806 or MBCDF-T). Two tumor microenvironment cell lineages, ECs (a) or SCs (b), were co-seeded with TNBC cells for 48 h, and the formation of tubular-like three-dimensional structures was observed only with ECs and TNBC co-cultures (a). Live cells were photographed, and representative bright field images are shown. Pictures are magnifications from 10× images, scale bar = 100 μm.