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. 2023 Sep 8;24(18):13865. doi: 10.3390/ijms241813865

Figure 3.

Figure 3

S1PR1 protein expression is increased in BLT mice systemically infected with dual-tropic HIV-1 and is abrogated by IFNAR2 blockade. (A) Schematic of infection of BLT mice with dual-tropic HIV (89.6) and analysis of thymic implants (n = 3–7 depending on treatment; n = 22 total). (B) S1PR1 expression is increased in two CD3/69 subsets in BLT mice infected with dual-tropic HIV-1 for 12 weeks. Expression was quantified by flow cytometry on CD3hiCD69+ and CD3hiCD69− thymocytes. Shown are three representative animals (mock, HIV and HIV + αIFNAR2) of a total n = 3 mock, n = 7 HIV-infected, n = 4 HIV + αIFNAR2-treated, n = 4 HIV + ART-treated, and n = 4 HIV + ART + αIFNAR2-treated mice. Gates for each condition were set based on isotype controls. (C,D) Summary of S1PR1 expression in CD3/69 thymic subsets in BLT mice infected with dual-tropic HIV-1 at 12 weeks post-infection. The mean with the standard error of the mean (SEM) is shown. Data were analyzed by one-way ANOVA using a Tukey HSD test for multiple comparisons. Overall, the data are statistically significant with p < 0.001.