Figure 2.

Superimposed tertiary structures of PvDFRa (pink), SbFNR1 (blue), and SbANR (green). Catalytic triad residues, as well as bound NADP⁺ are represented as sticks. The catalytic triads are shown with residue numbers and arrows. Additionally, Asn-148/Tyr-138/Ser-131 are important for substrate specificity. RMSD among those structures is shown in Figure S1. Molecular graphics images were produced using the ChimeraX package (UCSF).