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Reaction specificity of the putative ALOX15 ortholog of (A) N. furzeri and (B) its Val424Ile + Val425Met double mutant. Wildtype N. furzeri ALOX15 and its Val424Ile + Val425Met double mutant (cellular lysate supernatants) were incubated with AA in PBS for 3 min and the reaction products were analyzed by RP-HPLC.
