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. 2023 Sep 15;24(18):14154. doi: 10.3390/ijms241814154

Figure 9.

Figure 9

Reaction specificity of wildtype ALOX15 ortholog of N. furzeri and its Gly410Ala and Arg409Ile mutants. Enzyme preparations (cellular lysate supernatants of Sf9 cells) were incubated with AA in PBS for 5 min. The reaction products were reduced and analyzed by RP-HPLC as described in Materials and Methods. Insets: The conjugated dienes were prepared by RP-HPLC and further analyzed by combined NP/CP-HPLC (see Materials and Methods). (A) Wildtype enzyme, (B) Gly410Ala mutant, (C) Arg409Ile mutant.