Figure 1.

Tree no. 1 out of the four most parsimonious trees (length = 292) generated for positive samples with established Blastocystis sp. subtype and GenBank sequences of known subtype (accessions in bold). The consistency index is 0.845890 (0.763158), the retention index is 0.924115 (0.924115), and the composite index is 0.781700 (0.705245) for all sites and parsimony-informative sites (in parentheses). Clustering was assessed in the bootstrap test (1000 replicates; values > 80% are shown below the branches). The tree is drawn to scale, with branch lengths calculated using the average pathway method and in the units of the number of changes over the whole sequence. B1—first batch of samples, B2—second batch of samples.