Table 2.
List of primers used in this study. The identifier refers to the assigned library number for genes of interest given in [34,70], as well as gene accession number for the reference genes.
| Primer Name | Gene Name | Identifier | Primer Sequence fwd (5′→3′) | Primer Sequence rev (5′→3′) | Size (bp) | Efficiency % |
|---|---|---|---|---|---|---|
| Genes of interest [34] | ||||||
| NB_Chit6 | Chitinase 6 | k58:171433 | GATCGCTGCTTTCTTTGCTC | CGCCTGAAGGACCATTTATC | 80 | 95.69 |
| NB_Chn-A | Endochitinase A | k72:280831 | AACCTTCTTGCCACGATGTC | GTGATGACACCAAATCCAG | 91 | 92.96 |
| NB_GEβGluc | Glucan endo-1,3-beta-glucosidase | k60:395851 | GCTGCTTGTTGGGAGAAAAC | AGCCTGGACCTATTGAAACC | 102 | 95.17 |
| NB_PDRP1 | Pleiotropic drug resistance protein | k80:119278 | AGGTTTCATCGTTCCACGAC | AGGTCTCCGAATTGAGATGC | 111 | 92.55 |
| NB_PR-1a | Pathogenesis-related protein 1A | k58:459599 | TCCAACACGAACCGAGTTAC | TTGAGATGTGGGTCGATGAG | 116 | 81.84 |
| NB_PR-Q | Acidic endochitinase Q | k78:23474 | CCCCAGGAGCAACATTTAAC | AATGACGCAGTGGAAGATCG | 70 | 91.93 |
| NB_RNAP-β | DNA-directed-RNA polymerase subunit beta’ | k58:179615 | TCCTCTTATCCCAATCTGGTG | TTGACGGACACAAACTCTGC | 95 | 88.01 |
| NB_VAS | Lipid transfer-like protein | k64:395173 | TAGTCACGGTGGCGATTATG | GCGGTTTGGTGGAATTAAGG | 105 | 91.8 |
| NB_WIN2 | Wound-induced protein | k58:223872 | CCGTCAAAGGGTAAACATGG | GATGGAAGAGGGAATCAACG | 144 | 97.23 |
| NB_OEE1 | Oxygen-evolving enhancer protein 1 | k80:117138 | CCACATCATTCACGGTCAAG | TGCCATCAGAAGACACTTCG | 135 | 92.6 |
| NB_RuBisCO-BP | RuBisCO large subunit-binding protein subunit beta | k64:394932 | TACTGGCTTTTCCGTTCACC | TTGAGCAAGAAGCACTAGC | 103 | 88.00 |
| NB_SerThrKin | Serine/threonine-protein kinase | k78:183558 | TACCGATACCGTCCAATTCC | TGCACAGTCATGGTCTTG | 129 | 92.94 |
| NB_PSI-N | Photosystem I reaction centre subunit N | k76:226809 | GGCAGCAATGAACTCAAGTG | TGATTGGGAAGCCATAGAGG | 100 | 87.00 |
| Reference genes [70] | ||||||
| Nb_F-Box | F-box protein | Niben.v0.3.Ctg24993647 (At5g15710) |
GGCACTCACAAACGTCTATTTC | ACCTGGGAGGCATCCTGCTTAT | 127 | 100.8 |
| Nb_SAND | Sand family protein | Niben.v0.3.Ctg25188435 (At2g28390) |
ACCACCAACACCTATGAATGCT | CAGTCTCGCCTCATCTGGGTCA | 83 | 88.27 |
| Nb_L23 | 60S ribosomal protein | TC19271 (At2g39460) | AAGGATGCCGTGAAGAAGATGT | GCATCGTAGTCAGGAGTCAACC | 110 | 91.46 |
| Nb_UK | Uridylate kinase | EH363935 (At5g26667) | CTAGGAGTATATTGGAAGAGCG | AAAGATACATCGCCTTGCTGAA | 107 | 96.72 |
| Nb_GBP | GTP-binding protein | TC20872 (At5g59840) | GGAACTGGATTCGCAACATAGA | GACCCTTGGAAGTTGGCACAGC | 114 | 92.5 |
| Nb_RdR6 | Putative RNA-dependent RNA polymerase SDE1 |
AY722008 (At3g49500) | TTCAGGAATGTCTTCGAGCG | AGTGATCTAGCAACCCAATGAG | 134 | 93.3 |