Table 2.

Equilibrium Unfolding and refolding Parameters of wild type and F10_Y11delinsLN mutant γS-crystallin.

Protein	cm a	ΔG° (kcal mol−1) b	m (kcal mol−1 M−1) c
Wild type (UF)	2.72 ± 0.005	11.713 ± 0.490	4.310 ± 1.777
Mutant N↔I (UF)	1.25 ± 0.017	5.594 ± 0. 436	4.494 ± 0.345
Mutant I↔U (UF)	2.59 ± 0.008	8.409 ± 0.457	3.244 ± 0.179
Wild type (RF)	2.48 ± 0.011	7.318 ± 0.426	2.951 ± 0.168
Mutant N↔I (RF)	1.26 ± 0.039	4.805 ± 0.241	3.826 ± 0.198
Mutant I↔U (RF)	2.6 ± 0.020	7.873 ± 0.680	3.027 ± 0.271

^a Midpoint of GuHCl unfolding curve in M. ^b Free energy of unfolding in the absence of GuHCl in (kcal mol⁻¹). ^c Slope of GuHCl versus FI ratio in (kcal mol⁻¹ M⁻¹).