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. 2023 Sep 1;14(9):450. doi: 10.3390/jfb14090450
ESM Eggshell membrane
AgNPs Silver nanoparticles
C Commercially produced silver nanoparticles (Sigma-Aldrich)
M Green-synthesized silver nanoparticles (Metalchemy)
AgNPs-ESM Silver nanoparticles loaded eggshell membrane
A control Blank acetic acid extracted eggshell membrane
S control Manually peeled eggshell membrane
AC Acetic acid extracted ESM incorporated with commercial AgNPs
AM Acetic acid extracted ESM incorporated with Metalchemy AgNPs
SC Manually peeled ESM incorporated with commercial AgNPs
SM Manually peeled ESM incorporated with Metalchemy AgNPs
FM Filter membrane
VEGF Vascular endothelial growth factor
VEGF+FM FM loaded with 10 ng/mL vascular endothelial growth factor
SEM Scanning electronic microscopy
FTIR Fourier Transform Infrared Spectroscopy
UV Ultraviolet
CAM Chorioallantoic membrane
AA Acetic acid
HDFs Human dermal fibroblast cells
BJ Human fibroblast cells
FBS Fetal bovine serum
DMEM Dulbecco’s Modified Eagle’s Medium
PBS Phosphate-buffered saline
DI Deionized water
MTS assay CellTiter 96® AQueous One Solution Cell Proliferation assay
LDH assay CytoTox 96® Non-radioactive Cytotoxicity Assay
P/S Penicillin/streptomycin
EDTA Ethylenediaminetetraacetic acid
TCP Tissue culture plate
ANOVA Analysis of variance