5-HT1A receptor (5-HT1AR) (Abcam, Cambridge, MA, USA) |
1:500, 1:100 |
SNAP-25 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) |
1:1000, 1:100 |
PSD-95 (Novus Biologicals, Centennial CO, USA) |
1:2000, 1:100 |
Secondary antibody, horseradish peroxidase (HRP) anti-rabbit IgG (Cell Signaling Technology, Danvers, MA, USA) |
1:3000 |
Secondary antibody, HRP-linked anti-mouse IgG (Cell Signaling Technology, Inc., Danvers, MA, USA) |
1:3000 |
AlexaFluor-488-labeled goat anti-mouse IgG antibody (Invitrogen, Carlsbad, CA, USA) |
1:100 |
AlexaFluor-546 labeled goat anti-rabbit IgG antibody (Invitrogen, Carlsbad, CA, USA) |
1:100 |