TABLE 1.

Primers used for typing VT genes

Primer pair	Sequence (5′ to 3′)	Gene specificity	Location within gene	Size of amplified product (bp)	Reference or source
VT1-a VT1-b	GAA GAG TCC GTG GGA TTA CG AGC GAT GCA GCT ATT AAT AA	VT1	From nucleotide 860 of A-subunit-coding region to nucleotide 31 of B-subunit-coding region	130	Pollard et al. (30)
VT2-a VT2-b	TTA ACC ACA CCC ACG GCA GT GCT CTG GAT GCA TCT CTG GT	VT2, VT2vh-a, VT2vh-b, VT2d-Ount, VT2d-OX3a	From nucleotide 188 to nucleotide 533 of A-subunit-coding region	346	Pollard et al. (30)
VT2-c VT2-d	AAG AAG ATG TTT ATG GCG GT CAC GAA TCA GGT TAT GCC TC	VT2, VT2vh-a, VT2vh-b	From nucleotide 4 of B subunit region to nucleotide 21 of upstream region	285	Tyler et al. (38)
VT2v-1 VT2v-2	CAT TCA GAG TAA AAG TGG CC GGG TGC CTC CCG GTG AGT TC	VT2vh-a, VT2vh-b, VT2d-Ount, VT2d-OX3a	From nucleotide 110 of B-subunit-coding region to nucleotide 225 of upstream region	385	Tyler et al. (38)
VT2-e VT2-f	AAT ACA TTA TGG GAA AGT AAT A TAA ACT GCA CTT CAG CAA AT	VT2, VT2vh-a, VT2vh-b, VT2d-Ount, VT2d-OX3a	From nucleotide 883 of A-subunit-coding region to nucleotide 259 of B-subunit-coding region	348	This study
VT2-cm VT2-f	AAG AAG ATA TTT GTA GCG G TAA ACT GCA CTT CAG CAA AT	VT2d-Ount, VT2d-OX3a	From nucleotide 4 to nucleotide 259 of B subunit region	256	This study
VTe-a VTe-b	CCT TAA CTA AAA GGA ATA TA CTG GTG GTG TAT GAT TAA TA	VT2e	From nucleotide −25 downstream to nucleotide 205 of A-subunit-coding region	230	Johnson et al. (9)