TABLE 2.

Sizes of the restriction fragments used for RFLP analysis

Primers used to amplify fragment	Restriction enzyme	Size(s) of fragment(s) in genes coding for B subunit (bp)
		VT2	VT2c		VT2d
			VT2vh-a	VT2vh-b	VT2d-Ount	VT2d-OX3a
VT2-c, VT2-da	HaeIII	285	161, 124	161, 124
	RsaI	216, 69	136, 80 (69)b	216, 69
	NciI	285	285	159, 126
VT2-e, VT2-f	HaeIII	348	216, 132	216, 132	216, 132	167, 132 (49)b
	PvuII	323 (25)b	323 (25)b	250, 73 (25)b	200, 120 (28)b	200, 120 (28)b

^aThis primer pair was used according to the method of Tyler et al. (38). 

^bThis fragment did not resolve or was too small to be clearly visible under the electrophoresis conditions used.