FIG. 1.

Detection by PCR of AdV serotype 11 serially diluted in urine. AdV serotype 11 was propagated and quantified in A549 cells (see Materials and Methods). Serial 100- and 10-fold dilutions of this virus were made in urine from a healthy volunteer (AdV negative by culture and PCR). DNA from each dilution was column purified and then amplified by PCR. Controls for amplification were water (NEG) and 10² copies of AdV serotype 2 purified DNA (POS). (A) CCD-enhanced photography of an ethidium bromide-stained gel. (B) Autoradiogram of DNA in the gel from panel A after Southern blot transfer and hybridization with probe Hex 30 (20-h exposure).