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. 2023 Sep 18;24(18):14236. doi: 10.3390/ijms241814236

Figure 2.

Figure 2

Response of the innate microglial cells in blue LED-induced RD. Anti-IBA1 antibody (red) and anti-P2RY12 antibody (green) were used as markers for pan-microglial cell and innate microglial cell populations, respectively. (A–E). Representative images of IBA1/P2RY12-double-labeled vertical sections taken from normal retina (A) and RD retinas at 12 (B), 24 (C), 72 (D), and 120 h (E). Each boxed area is magnified in (A’–E’), respectively. Scale bars: 50 μm (A–E), 10 μm (A’–E’). (F–I). Quantitative analyses of the IBA1/P2RY12-double-labeled microglial cells in RD. The cell number was counted in the range of 700 μm from the optic disc in retinal vertical sections and presented as cell number per mm by each layer: whole retina (F), ONL (G), OPL to GCL (H), and SRS (I) (n = 5). Data are presented as the mean ± S.E.M. * p < 0.05, one-way ANOVA with Tukey’s multiple comparison post-hoc test, ** p < 0.05, Tukey’s multiple comparison test. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; SRS, subretinal space.