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. 2023 Sep 19;24(18):14263. doi: 10.3390/ijms241814263

Figure 2.

Figure 2

MtbHLHm1;1 influences mycorrhizal colonisation in a Pi-dependent manner. (A) Gene expression of MtbHLHm1;1 in transgenic (empty vector) non-inoculated roots at two Pi (phosphate) levels: 50 and 500 µM. (A) Gene expression analysis by RT-qPCR. Values indicate fold-change in expression relative to UBQ10 [49]. (B–D) AM colonisation response (%MC) in mycorrhizal roots of empty vector control (Control) and 35Spro::MtbHLHm1;1 (RNAi) grown at 50 and 500 µM Pi. (B) Mycorrhizal colonisation (%) in colonised roots (MC%); (C) Arbuscule abundance (%) in colonised roots; (D) Degraded arbuscules (%) in colonised roots. (E) Representative mycorrhizal empty vector control (Control) and 35Spro::MtbHLHm1;1 (RNAi) roots stained with 5% ink grown at two Pi levels 50 and 500 µM. Scale bars (E): 200 µm. Data in (A–D) represent the mean ± SEM. Significance (p < 0.05, p < 0.001) was determined using a t-test (A, n = 12 plants) or two-way ANOVA (B–D, n = 5–8 plants) with a multiple comparison test (Sidak).