FIG. 2.

RT-PCR detection of LAC virus RNA in CNS tissues of a patient from 1978. Total cellular RNA was extracted from the medulla (M), cerebral cortex (CO), cerebellum (CE), spinal cord (SC), basal ganglion (BG), or temporal lobe (T), each containing two sets of tissues subjected to RT-PCR with primers specific for viral genomic, mRNA, or vcRNA as described for Fig. 1. V+ and V− are control reaction mixtures containing RNA extracted from BHK-21 cells inoculated with LAC virus (V+) or from noninfected BHK-21 cells (V−). P and W, plasmid (positive) and water (negative) PCR controls. m, molecular weight markers (123-bp DNA ladder). Numbers in white are base pairs.