TABLE 1.

Primers for RT-PCR of LAC virus S RNA

RT primer	Sequence (5′→3′)	Bases represented	Paired primer for PCR	LAC virus RNA detected	Product size (bp)
LNF	TCAAGAGTGTGATGTCGGATTTGG	71–95 of LAC S vcRNA	LVC	S genome	860
LNR	GGAAGCCTGATGCCAAATTTCTG	763–785 of LAC S genomic RNA	LNF	S mRNA and vcRNA	715
LVC	TTTTGCTGTCCCCTACCACC	911–931 of LAC S genomic RNA	LNF	S vcRNA	860
LNF2	CAAATTCTACCCGCTGAC	550–568 of LAC S genomic RNA	LNR	S genome	214
LNR 2	ATGGTCAGCGGGTAGAAT	553–571 of LAC S genomic RNA	LNF	S genome	482