TABLE 2.

Detection by PCR of genomic groups of B. burgdorferi s.l. in Ixodes ticks

Areaa	Stageb	Tick infection ratec		No. of ticks with spirochetes in genomic groupd:
		No. of ticks tested	No. positive (%)	Ss	Ga	Af
Co	N	97	18 (19)	14	14	0
	A	8	3 (38)	1	3	0
Subtotal		105	21 (20)	15	17	0
F	N	116	32 (28)	16	22	4
	A	12	0 (0)
Subtotal		128	32 (25)	16	22	4
Ca	N	109	24 (22)	11	17	6
	A	23	10 (43)	9	10	2
Subtotal		132	34 (26)	20	27	8
A	N	122	25 (20)	5	13	2
	A	2	2 (100)	0	0	0
Subtotal		124	27 (22)	5	13	2
Total		489	114 (23)	56	79	14

^aCo, Condroz; F, Fagne; Ca, Calestienne; A, Ardenne. 

^bN, nymph; A, adult. 

^cInfection rate determined by PCR with the primers of Rosa et al. (30). 

^dGenomic groups were determined by PCR with species-specific primers (18). Ss, B. burgdorferi s.s.; Ga, B. garinii; Af, B. afzelii.