Figure 2.

ADPN increases SK2 phosphorylation in murine gastric fundus. A fragment of stomach was treated with ADPN (20 nM) for 20 min before being lysed as described in the Methods Section (Section 2). Western blot analysis was performed using (a) specific anti-phospho-SK1 (Ser225) (P-SK1) and (b) anti-phospho-SK2 (Thr578) (P-SK2) antibodies. A blot representative of two independent experiments with analogous results is shown. The bar chart represents densitometric analysis of two independent experiments. Data are the mean ± SEM and are reported as protein expression normalized to GAPDH, fold change over control (set as 1). a.u.: arbitrary units. The increase in P-SK2 content induced by ADPN was found to be statistically significant by t test (* p < 0.05, treated vs. CTRL).