Table 1.
Chromatographic gradient.
| Time | Flow (mL/min) | Mobile Phase A | Mobile Phase B |
|---|---|---|---|
| 0.01 | 0.3 | 95 | 5 |
| 3.00 | 0.3 | 95 | 5 |
| 4.50 | 0.3 | 38 | 62 |
| 5.50 | 0.3 | 38 | 62 |
| 7.50 | 0.3 | 25 | 75 |
| 8.00 | 0.3 | 25 | 75 |
| 8.50 | 0.3 | 0 | 100 |
| 9.00 | 0.3 | 0 | 100 |
| 9.50 | 0.3 | 95 | 5 |
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