FIG. 1.

SDS-PAGE analysis of affinity-purified GST-VCA fusion protein. The induced bacterial cells containing fusion protein were lysed by 0.1% Triton X-100 detergent in Tris-HCl buffer with mild sonication, clarified by centrifugation at 10,000 × g for 15 min, and purified by GST-glutathione affinity chromatography with glutathione-Sepharose 4B. The bound GST fusion proteins were eluted with glutathione elution buffer. The samples were loaded onto an SDS–10% polyacrylamide gel and stained with 0.5% Coomassie blue to visualize the fusion protein and control parental GST protein (made in control cells carrying the parental pGEX vector only). Masses are shown on the left, in kilodaltons.